More proteins were detected on the three overlapping gels than on the single 3—10 pH range gel. If you are an educator at the high school or college level, visit our Education Discount Policy page to establish an education account number. Remove any large bubbles trapped under the IPG strip after rehydration solution is applied. It is the total Number of volt hrs that you have caried out yr focussing on is important irrespective of the total duration. Its the total number of volt hours that are important and not the final voltage reached.